South Norfolk MP Richard Bacon described the EEAST as "lions being led by donkeys" and called for a "clear out" following a damning report by health watchdog the Care and Quality Commission (CQC).
It gave the ambulance trust a week to address the concerns and to come up with an action plan.
He said: "Other ambulance trusts are facing considerable pressures and are doing a better job."
Yesterday the EEAST declined to comment on Mr Freeman's remarks.
The trust is holding a board meeting on Wednesday in Cambourne in Cambridgeshire where the concerns raised by MPs and the CQC over leadership and the service given to patients are expected to be addressed.
Management is attempting to improve the service by recruiting more paramedics and a month ago it introduced 15 extra ambulances although, as reported in the EDP, not all of these ambulances are fully staffed.
Despite the EEAST's leadership pledging improvements response times in Norfolk are deteriorating.
Mid Norfolk MP George Freeman became the third MP in the region to call on members of the East of England Ambulance Service Trust (EEAST) board to consider their positions.
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Yesterday North West Norfolk MP Henry Bellingham stopped just short of backing Mr Freeman's call but said the board had a "limited amount of time to deliver".
Ms Ball also revealed in the email that Mr Morgan, who joined the trust in December, had been given a 12 month contract extension.
Ms Ball has refused to speak to the EDP but in a joint statement with chief executive Andrew Morgan she said: "It is a journey ahead of us, not a sprint. You will see some immediate changes and some will take longer, but it should start to feel different quickly.
But on Friday Maria Ball, who has chaired the trust for 11 years, sent a defiant email to staff in which she pledged to make improvements.
He said: "Patients, taxpayers and nurses and doctors have a right to expect well paid NHS managers to be accountable for failures and take responsibility.
He told the EDP: "I think Maria Ball needs to get a grip. On her watch this service has gone downhill.
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The remarks from the Conservative MPs come after two other politicians questioned the future positions of the trust's board members last week.
be accountable for its failings.
fire ambulance service ahead of board meeting
"If her and her co directors do not get a grip very soon, it is time for them to go."
Mr Morgan replaced the former chief executive Hayden Newton who announced his retirement three days after the EDP's Ambulance Watch campaign was launched.
It found that response times had worsened in the last 12 months in Norfolk and stated that management appeared to have few answers to the trust's problems.
Suffolk Costal MP Therese Coffey also said that the board's directors and chairman should reconsider their positions.
"Our ambulance service has been found by an independent regulator to be failing to meet the necessary standard. As with Stafford and David Nicholson, someone must take responsibility. "
Mr Freeman Nike Zoom Kd 9 Black Space said "heads must roll" at the trust and called for someone at the service to Nike Kyrie 2 White Metallic Silver
The CQC report found that the service was failing to deliver on "care and welfare of people who use the service".
be the best ambulance trust again."
The failings of the service have been repeatedly highlighted through the EDP's Ambulance Watch campaign, which began last October to keep a watch on our region's ambulance service.
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